Journal: IBRO Neuroscience Reports
Article Title: Trimethylamine-N-oxide damages astrocytes and lymphatic endothelial cells in the cerebral lymphatic system
doi: 10.1016/j.ibneur.2025.09.001
Figure Lengend Snippet: Effects of TMAO on intracranial inflammation and functional proteins in animals. Levels of IL-6, NLRP3, TNF-α, and SNCA in the cerebrospinal fluid detected by ELISA. (B) The injury of ventral midbrain neurons detected by Nissl staining. (C) Immunofluorescence was used to detect the effects of TMAO on AQP4, GFAP, S100, claudin-5, and Ocln in the ventral midbrain of mice. Three animals were randomly selected from each group (n = 10) for sample testing, analysis of variance followed by LSD or Dunnett's post-hoc test was used to compare between-group differences. 'ns' indicates that there was no significant difference between the groups.
Article Snippet: The primary antibodies used include S100 (1: 100, bs-1248R, Bioss, China), glial fibrillary acidic protein (GFAP, 1: 100, R380620, Zenbio, China), AQP4 (1: 200, A11210, ABclonal, China), claudin-5 (1: 200, A10207 , ABclonal, China), and Ocln (1: 200, YN2865, Immunoway, China), and the secondary antibody was goat anti-rabbit IgG (1: 500, 550076, Zenbio, China).
Techniques: Functional Assay, Enzyme-linked Immunosorbent Assay, Staining, Immunofluorescence